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| Registros recuperados: 16 | |
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| Torres,Giovana A.; Parentoni,Sidney N.; Lopes,Maurício A.; Paiva,Edilson. |
| The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregant analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotypic measure of tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300017 |
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| Maki,Cristina Sayuri; Teixeira,Flavia França; Paiva,Edilson; Paccola-Meirelles,Luzia Doretto. |
| The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Edible mushroom; RAPD; Mycelial growth. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300002 |
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| Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H.. |
| The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insect pathology; Fall armyworm larvae; PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010 |
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| Pinto,Patrícia P.; Raposeiras,Ruy; Macedo,Andrea M.; Seldin,Lucy; Paiva,Edilson; Sá,Nadja M.H.. |
| High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45ºC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bean nodulating Rhizobium strains; High temperatures. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400012 |
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| Santos,Cíntia Guimarães dos; Paiva,Renato; Paiva,Patrícia Duarte de Oliveira; Paiva,Edilson. |
| Conduziu-se este trabalho com o objetivo de estudar o efeito de 2,4-D e BAP na indução da formação de calos originados de segmentos foliares de plântulas de Coffea arabica L., cv Rubi, obtidas in vitro, a partir da cultura de embriões, determinar sua curva de crescimento e analisar bioquimicamente, mediante determinação dos teores de proteínas totais, açúcares redutores e padrão proteíco. Segmentos foliares inoculados em meio desprovido de reguladores ou na presença de BAP em condições isoladas não apresentaram formação de calos. A produção máxima de calos é obtida utilizando-se 1,0 mg L-1 de 2,4-D. A curva de crescimento de calos apresenta crescimento tipo sigmóide, com três fases distintas: lag (entre 0 - 42 dias de inoculação), exponencial (42 - 77... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Indução de calos; Padrão protéico. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000300011 |
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| Rabel,Marcelo; Vieira,Elisa Serra Negra; Lana,Ubiraci Gomes de Paula; Paiva,Edilson; Sehnem,Margarete Aparecida Sella; Schuster,Ivan. |
| Marcadores microssatélite têm sido utilizados na determinação da pureza genética em programas de melhoramento e de produção de sementes, visando garantir a qualidade genética das sementes. Objetivou-se neste trabalho demonstrar, com auxílio de marcadores moleculares microssatélites, que variações na coloração do hilo em sementes de soja, muitas vezes não constituem variação genética. Foram avaliadas sementes de soja da cultivar CD 222 que apresenta sementes com hilo preto, e de duas linhagens (CD 02RV-8444 e CD 01RV-7618), as quais apresentam sementes com hilo marrom. As sementes com variações na coloração e tonalidade do hilo encontradas em cada genótipo foram separadas visualmente em grupos. O DNA de cada semente foi extraído e analisado com marcadores... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Cor do hilo; Pureza genética; SSR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222010000200002 |
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| Drummond,Rodrigo D.; Guimarães,Claudia T.; Felix,Juliana; Ninamango-Cárdenas,Fernando E.; Carneiro,Newton P.; Paiva,Edilson; Menossi,Marcelo. |
| Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100029 |
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| Raposeiras,Ruy; Marriel,Ivanildo Evódio; Muzzi,Maria Rita Scotti; Paiva,Edilson; Pereira Filho,Israel Alexandre; Carvalhais,Lilia Costa; Passos,Raul Vinícius Magalhães; Pinto,Patrícia Pereira; Sá,Nadja Maria Horta de. |
| The objective of this work was to identify the most competitive and effective Rhizobium strains in order to increase common bean yield by nitrogen fixation as alternative or complementation to the nitrogen fertilization. Competitiveness tests were lead in axenic conditions, in Cerrado soil pots and in three field experiments, with native Rhizobium strains that were previously identified, according to their effectiveness and genetic variability. The identification of strains in nodules was performed using serological tests (axenic conditions) - agglutination and enzyme linked immunosorbent (Elisa) assays - and random amplified polymorfic DNA (RAPD) (Cerrado soil). Plant yield was determined using the dry weight (greenhouse conditions), total N and grain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Nodule occupancy; Elisa; RAPD. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000300010 |
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| Raposeiras,Rui; Pinto,Patrícia P.; Passos,Raul V.M.; Seldin,Lucy; Paiva,Edilson; Scotti,M. Rita; Sá,Nadja M.H.. |
| Irregular response to bean plants to Rhizobium inoculation has been attributed to among other factors, low competitive ability, low N2 fixation efficiency and genetic instability of the symbiont. This genetic instability caused by high rates of genomic rearrangements and/or plasmid deletions can be accentuated by high temperatures. This fact may limit the utilization of these strains as inoculants, especially in tropical soils. In this study, the variability of isolated colonies derived from effective R. leguminosarum bv. phaseoli (SLP1.3 and BR 10.026) and R tropici (SLA2.2 and BR322) strains was evaluated before and after exposure to high temperatures (four consecutive thermal shocks at 45ºC). This evaluation involved plant dry matter analysis of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizobium tropici; Rhizobium leguminosarum bv. phaseoli; Bean plants; High temperature; Cerrado soils. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200010 |
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| Registros recuperados: 16 | |
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